Genetic variability among elite red clover ( Trifolium pratense L.) parents used in Chile as revealed by RAPD markers

Red clover (Trifolium pratense L.) is one of the main forage species of temperate regions. Cultivars of red clover are heterogeneous which makes their genetic analysis difficult. We applied RAPDs (Random Amplifed Polymorphic DNA) in order to assess the genetic relationship and levels of genetic variability existing among a group of 16 elite red clover parents organised in four subsets of 4 parents each. Out of 55 primers 21 provided reproducible results. A total of 135 reliable and polymorphic RAPD bands were detected which were used to estimate genetic distances among pair-wise combinations of elite parents. Nei and Li's similarity values ranged from 0.60 to 0.77, with a mean of 0.66, which reflects a rather high genetic variability among the genotypes evaluated. Lower levels of genetic variability, as detected by polymorphic loci and mean heterogeneity values, were detected in a subset of parents selected for resistance to the stem nematode. Cluster analyses resolved the different sets of parents in a manner consistent with what is known from their breeding origins. An Analysis of Molecular Variance detected substantial levels of variation within subsets of parents. RAPDs represent a valuable source of genetic information for red clover breeding programmes. This revised version was published online in August 2006 with corrections to the Cover Date.     

Intra-varietal variation for in vitro plant regeneration in the genus Trifolium

Summary Seedlings of Trifolium repens showed considerable variation with regard to the morphology and growth of their calli, and their ability for in vitro differentiation of shoots. One of the lines selected for regeneration in primary callus cultures also showed shoot formation from protoplasts. Somatic embryogenesis in callus cultures of T. pratense and T. arvense occurred only in selected seedling lines. This paper highlights the importance of screening a large number of plants within a cultivar of outbreeding species to achieve reproducible plant regeneration from tissue culture.     

不同氨基酸组合对真鲷(Chrysophrys major)诱食效果的研究

试验以真鲷为试验对象,采用迷宫法,以丙氨酸、甘氨酸、精氨酸和组氨酸作为诱食剂原料,经不同组合后测定其对真鲷的诱食效果。结果显示,甘氨酸+组氨酸的诱食效果最好,其各种组合的诱食效果依次为甘氨酸+组氨酸>甘氨酸+丙氨酸>甘氨酸>组氨酸>丙氨酸>丙氨酸+精氨酸>丙氨酸+甘氨酸+组氨酸>精氨酸+组氨酸>甘氨酸+精氨酸>甘氨酸+丙氨酸+精氨酸>丙氨酸+组氨酸>精氨酸>丙氨酸+精氨酸+组氨酸>甘氨酸+丙氨酸+精氨酸+组氨酸。     

陕西渭北旱源地区葡萄节水施肥与栽培技术研究

介绍了在海拔700～1000m高度且无灌溉条件的陕西渭北旱塬地区葡萄的节水施肥和"V"形架栽培技术,并将其与水肥条件良好的平原地区做了比较。结果表明:在陕西渭北旱塬地区使用黑地膜覆盖技术和施肥枪施肥技术,不但节约水肥,而且经济效益显著,值得推广应用。     

Effect of hot air,UV-C,white light and modified atmosphere treatments on expression of chlorophyll degrading genes in postharvest broccoli (Brassica oleracea L.) florets

Several treatments were applied in order to delay postharvest degreening in broccoli florets and investigate their effects on genes associated with chlorophyll catabolism. Degradation of chlorophylls is the most evident visual manifestation of broccoli postharvest deterioration, occurring rapidly due to the immature stage in which the material is harvested. Treatments such as storage in modified atmosphere, exposure to hot air, UV-C and white lamps were employed in the current work to induce a delay in degreening and chlorophyll degradation. Expression of genes possibly related to chlorophyll catabolism was analyzed in these samples and discussed. Chlorophyllases, the enzymes traditionally believed to remove the phytol side chain from chlorophyll appear to have a gene expression that was not regulated by postharvest treatments. Pheophytinase, a recently discovered new enzyme in this metabolic pathway, correlated chlorophyll loss accurately in heat, UV-C and white-light treatments, but not in modified atmospheres. Results presented in this work indicate that postharvest treatments that delay chlorophyll degradation have a higher effect on the expression of pheophytinase rather than on chlorophyllase genes.     

红梨新品种‘红月梨’

 ‘红月梨’是由‘红茄梨’和‘苹果梨’杂交育成。果实圆锥形,平均单果质量245 g。表面红色,果皮薄。果肉白色,后熟后肉质细腻多汁,风味酸甜,微香,石细胞少,含可溶性固形物14.4%,总糖10.33%,可滴定酸0.34%,维生素C 0.0248 mg · g-1,品质优。在辽宁熊岳地区9月上旬成熟,早产,早丰,优质,易栽培管理,抗病,抗寒。     

Ecological Benefit of Reforestation in a Severely Degraded Red Soil Region

Guifeng, Yiyang County, belongs to a seriously degraded red soil region. In 1991, Pinus elliottii and Lespedaza spp. were the species selected for the establishment of a mixed forest in the area. The results of an investigation of the soil system in a 12-year-old forest indicated the following: (1) Organic matter and total nitrogen of the forest soil to a depth of 40 cm were 88.0 and 36.0% higher, respectively, than those of a control plot; total phosphorus and available phosphorus were 40.9 and 22.3% higher than those of the control; available potassium contents were 8.13% lower than those of the control. (2) Soil aeration and the soil air regime improved. (3) Proteinase, catalase, and urease in the forest soil to a depth of 40 cm were usually higher than those in the control plot and decreased with soil depth. Translated from Acta Agriculturae Universitatis Jiangxiensis, 2005, 27(1) (in Chinese)     

湿地松幼林施肥五年生长反应

在江西省永丰县恩江林场贫瘠的第四纪低丘红土立地进行湿地松林施肥试验，用以检验要对Ｎ、Ｐ、肥效的反应，和不同施肥期对幼林生长的影响，施肥后五年的生长统计分析表明：Ｎ、Ｐ配合施用效果最佳；施用量为Ｎ１００ｋｇ／ｈｍ＾２、Ｐ２Ｏ５１００ｋｇ／ｈｍ＾２时，立木蓄积达到４２．０１ｍ＾３／ｈｍ＾２，比不施肥增加９０％；单施肥湿地松幼林的生长反应也达到极显著水平。造林施Ｐ２Ｏ５５０－２００ｋｇ／ｈｍ＾２时，立木     

涨南红豆红锈病的发生与危害

海南省尖峰岭地区营造的5－6年生海南红豆行道树，严重发生了由红锈藻引起的红锈病，在500株幼树中，发病株率高达97.5%，Ⅲ-Ⅳ级病株率80％，病情指数75.5，导致部分叶片干枯或脱落，嫩枝枯死，树势衰弱，生长严重受阻。文章对该病的症状、病原、危害及发生规律进行了报道。     

Dormancy induction in container-grown Abies seedlings: Effects of environmental cues and seedling age

Twelve-week-old container-grown seedlings of noble fir (Abies procera Rehd.) and Shasta red fir (A. magnifica A. Murr. var. shastensis Lemm.), both high-elevation species, were grown under controlled environments in a study of induction of terminal-bud dormancy. Eight treatment combinations of long (15 h) or short (11 h) photoperiods, warm (25°/20°C) or cool (18°/12°C) thermoperiods, and dry (–1.2 MPa) or wet (–0.6 MPa) moisture regimes were imposed upon seedlings for 12 weeks. Treatment factors significantly affecting the induction of dormancy in terminal buds of seedlings were identified over time. The results suggest that seedlings responded dynamically to dormancy cues. The warm/dry and cool/wet combinations induced dormancy in the first 2 weeks in noble fir and in the first week in Shasta red fir. Short-day/warm and long-day/cool combinations enhanced dormancy induction in weeks 3 to 4 in noble fir and in weeks 1 to 7 in Shasta red fir. Short days and cool thermoperiods independently hastened dormancy induction in noble fir in weeks 5 through 12.